SARS-CoV-2 XpressBio Research Reagents

A White Paper on Serology

COVID-19

Outbreaks with a novel coronavirus disease COVID-19 is caused by the SARS-CoV-2 virus. The virus has shown to be very contagious and has caused a world wide pandemic in the spring of 2020. The SARS-CoV-2 virus is a single strand RNA virus with glycoprotein spikes on its surface.  The spike protein (S) contains the receptor-binding domain (RBD) which has affinity to the angiotensin-converting enzyme 2 (ACE2). The interaction between RBD and ACE2 mediates the fusion of the viral and cellular membranes starting the infection process. The S protein has two subunits, S1 and S2. S1 contains the RBD region and the S2 is associated with the membrane of the virus. The nucleoprotein (N) is an abundant protein associated with virus structure and replication.

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SARS-CoV-2 Research Reagents

 

Microplates

Controls

Antigens

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SARS-CoV-2 S1 & S2

Ref# SP864C : SARS-CoV-2 S1/S2 (Wuhan Hu-1) Microplate.

The antigen is a mixture of recombinant SARS-CoV-2 Spike glycoprotein (S1) and SARS-CoV-2 Spike glycoprotein (S2).


Ref# SPC864 : SARS-CoV-2 S1/S2 Positive Control (Mouse monoclonal) 

 

Ref# 595-200 : SARS-CoV-2 Negative Control.

 

Ref# XCOV006 : SARS-CoV-2 Recombinant Spike Glycoprotein (S1) produced in HEK293 cells. Sequence strain Wuhan-Hu-1, amino acids 1-674, SHFc-Tag.

Ref# XCOV007 : SARS-CoV-2 Recombinant Spike Glycoprotein (S2) produced in HEK293 cells. Sequence strain Wuhan-Hu-1, amino acids 685-1211, SHFc-Tag.

Ref# SP869C : SARS-CoV-2 Spike Protein (Omicron) Microplate.

The antigen is a recombinant protein produced in HEK293 cells derived from the Omicron strain. 

Ref# XCOV020 : SARS-CoV-2 Recombinant Spike Protein produced in HEK293 cells. Description: Full length trimeric spike protein (strain B1.1.529/Omicron) produced in HEK293 cells with His-Strep tag. The protein contains A67V, H69del, V70del, T95I, G142D, V143del, Y144del, Y145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F mutations relative to the Wuhan Hu-1 strain.

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SARS-CoV-2 RBD + M-Protein

Ref# SP866C : SARS-CoV-2 RBD + M Protein Microplate

The antigen is a recombinant SARS-CoV-2 Fusion Protein RBD (234 aa) and M-Protein.


Ref# SPC866 : SARS-CoV-2 RBD + M Protein Positive Control (Chimeric monoclonal antibody with Human constant IgG1 domain). 

 

Ref# 595-200 : SARS-CoV-2 Negative Control.

 

Ref# XCOV001 : SARS-CoV-2 Recombinant Fusion Protein, Receptor-Binding Domain (RBD) and M-Protein (Virion Surface Domain of Membrane Protein) produced in HEK293 cells. RBD protein, 234 amino acids, C-His-Tag.    

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SARS-CoV-2 NUCLEOPROTEIN

Ref# SP865C : SARS-CoV-2 Nucleoprotein Microplate.

The antigen is a recombinant SARS-CoV-2 Nucleocapsid protein.


Ref# SPC865 : SARS-CoV-2 Nucleoprotein Positive Control (Mouse monoclonal) 

 

Ref# 595-200 : SARS-CoV-2 Negative Control.

 

Ref# XCOV002 : SARS-CoV-2 Recombinant Nucleocapsid protein produced in E. coli, clone nCOVNA. N-protein, amino acids 1-419, His-Tag.

Ref# XCOV003 : SARS-CoV-2 Recombinant Nucleocapsid protein produced in E. coli, clone nCOVNB.

N-protein, amino acids 1-419, Fusion protein.

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Human Coronavirus

Ref# SP867C : HCoV-229E Microplate

The antigen is a recombinant nucleocapsid protein (359 aa).

 

Ref# SP868C : HCoV-NL63 Microplate

The antigen is a recombinant nucleocapsid protein (aa 221-340).

 

 

Ref# 595-200 : SARS-CoV-2 Negative Control.

 

Ref# XCOV008 : HCoV-229E Recombinant nucleocapsid protein produced in E. coli. 359 aa, C-His-Tag

Ref# XCOV009 : HCoV-NL63 Recombinant nucleocapsid protein produced in E. coli. Aa 221-340, C-His-Tag

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A White Paper on Serology

The white paper describe assessment of sensitivity and specificity of a multi-plate antigen strategy by testing Non-Human Primates.

We would suggest testing the samples on the S1 and S2 spike plates first. If a sample is positive, the same sample could be tested on the nucleoprotein plate, RBD + M plate and the seasonal coronavirus plates. If the sample is positive on the spike plate and negative on the others, it would indicate that the sample has antibodies to the HEK vector or the Fc tag as the others are different vectors and tags. If the sample is positive on all, it could indicate a cross reaction to other coronavirus, otherwise it is a non-specific sera. 

Please Download the White Paper here

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Other Reagents
HIV p24 ELISA
Spike Fusion Protein
 in Vaccine and Pathogenesis Research
Rat and Mouse Controls

Ref# 595-711 : Mouse SARS-CoV-2 Nucleoprotein Positive Control

Ref# 595-712 : Mouse SARS-CoV-2 S1/S2 Positive Control

Ref# 595-713 : Rat SARS-CoV-2 Nucleoprotein Positive Control

Ref# 595-714 : Rat SARS-CoV-2 S1/S2 Positive Control

A recent study in nonhuman primates shows that the use of a recombinant spike fusion protein, S1Fc, on the virus, prevents infection by inhibiting the fusion of the virus to the host cell membrane.

In other words, this protein antigen is a highly immunogenic molecule and stimulates the production of high levels of neutralizing antibodies. The Researchers tested the immune resistance recombinant SARSCoV2 S1Fc fusion protein in mice, rabbits, and monkeys against SARSCOV2 as a potential vaccine candidate.

www.news-medical.net article

Other kits (Human)
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Bibliography
Human ACE2 ELISA Kit

XpressBio has developed a Human ACE2 ELISA Assay to help with SARS-CoV-2 research.

 

ACE2 is a transmembrane enzyme shown to aid in the entry of SARS CoV-2 into cells. Understanding the mechanism between SARS-CoV-2 and ACE2 is critical to developing effective long-term treatments for COVID-19.

Our Human ACE2 ELISA Assay can be used to quantify human ACE2 in cell culture media, cell culture lysate, plasma, or serum. We want to help you with your research.

Ref# ACE2-1000 : Human ACE2 ELISA Assay

Package Insert

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648506/pdf/main.pdf

Acute Respiratory Distress in Aged, SARS-CoV2-Infected African Green Monkeys but Not Rhesus Macaques

 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7361642/

SARS‐CoV‐2 surveillance for a non‐human primate breeding research facility

RNA Lysis Buffer

Xpress RNA Lysis Buffer is intended for the isolation and purification of total nucleic acids (DNA/RNA) from
biological specimens for research use.


Xpress RNA Lysis Buffer is designed for:


• Nasopharyngeal swab samples
• Lysis of DTT pre-treated sputum samples which

  had been spun down to pellets before adding the RNA Lysis buffer
• Stabilization of nucleic acids with lysates
• Nucleic acid purification using magnetic beads

Ref# LB-2X500   Xpress RNA Lysis Buffer, 2X (500ml)    

Ref# LB-2X10L   Xpress RNA Lysis Buffer, 2X (10L)  

 

Data Sheet 

Price list is at the Downloads page. Products for Research Use Only.