ImmunoFluorescence (BioDoc production)

Prof. Dr. Michael Mähler is owner and managing director of BioDoc in the Medical Park Hannover.

His professional experience is further reflected by teaching assignments at Medical School Hannover and RWTH Aachen University, preparation of peer reviews for scientific journals and active participation in several scientific organisations.

Pathogens

Species

Catalog Ref.

BioDoc IFA slides

MNV

Mouse Parvovirus (MPV)

Mouse hepatitis virus (MHV) / Rat coronaviruses (using MHV as antigen)                 

Reovirus type 3 (Reo3)                                                                                              

Theiler’s murine encephalomyeltitis virus (TMEV) / Rat theilovirus (using TMEV as antigen)  

Pneumonia virus of mice (PVM)                                                                            

Sendai virus (Sendai)                                                                     

Minute virus of mice (MVM)                                          

Ectromelia virus (Ectro)                                                           

Lymphocytic chroriomeningitis virus (LCMV)                             

Mouse adenovirus type 1 (K87)                                        

Mouse adenovirus type 2 (FL)                                      

Guinea pig adenovirus (detected by using K87)           

Simian Virus 5 (SV5)                                                      

Rotavirus (EDIM)                                                             

Rat parvoviruses (RPV) (detected by using Kilham rat virus as antigen)  

Myxomatosis virus                                                     

Guinea pig cytomegalovirus (CMV)                                     

Parainfluenza virus type 3 (PI3)                                         

Murine norovirus (MNV)

Hantavirus     New

Mycoplasma pulmonis     New

                                                 

M

M / R  

M, R, H, Gp, Rb

M, Gp / R

M, R, H, Gp, Rb

M, R, H, Gp, Rb

M

M

M, H, Gp

M, R

M, R

Gp

H, Gp, Rb

M, Rb (not rats!)

R

Rb

Gp

Gp

M

M, R

M, R, H, GP

 

M = mouse

R = rat

H = hamster

Gp = guinea pig

Rb = rabbit

IFA-MPV-S102

IFA-MHV-S202 / IFA-MHV-S202R

IFA-Reo3-S203

IFA-TMEV-S204 / IFA-TMEV-S204R

IFA-PVM-S205

IFA-Sendai-S206

IFA-MVM-S207

IFA-Ectro-S208

IFA-LCMV-S209

IFA-K87-S210

IFA-FL-S211

IFA-K87-S212

IFA-SV5-S213

IFA-Rota-S214

IFA-KRV-S215

IFA-Myxo-S216

IFA-GPCMV-S217

IFA-PIV3-S218

IFA-MNV-S219

IFA-HANT-S220

IFA-PULM-S221

Reo3
K87
Ectro
MHV
TMEV
SV5

SV5

MVM
Sendai

BioDoc IFA Positive controls

Reference #

IFA-PC102

IFA-PC202M

IFA-PC203M

IFA-PC204M

IFA-PC205M

IFA-PC206M

IFA-PC207M

IFA-PC208M

IFA-PC209M

IFA-PC210M

IFA-PC211M

IFA-PC213M

IFA-PC214M

IFA-PC220M

IFA-PC221M

IFA-PC219M

IFA-PC202R

IFA-PC203R

IFA-PC204R

IFA-PC205R

IFA-PC206R

IFA-PC209R

IFA-PC210R

IFA-PC211R

IFA-PC220R

IFA-PC215R

IFA-PC221R

IFA-PC203H

IFA-PC205H

IFA-PC206H

IFA-PC203GP

IFA-PC204GP

IFA-PC205GP

IFA-PC206GP

IFA-PC210GP

IFA-PC217GP

IFA-PC218GP

IFA-PC203RB

IFA-PC205RB

IFA-PC206RB

IFA-PC213RB

IFA-PC214RB

IFA-PC216RB

Infectious Agent / Disease

MPV IFA Positive Control, Mouse

MHV Positive Control, Mouse

Reo-3 Positive Control, Mouse

TMEV Positive Control, Mouse

PVM Positive Control, Mouse

Sendai Virus Positive Control, Mouse

MVM Positive Control, Mouse

Ectromelia Virus Positive Control, Mouse

LCMV Positive Control, Mouse

MAD K87 Positive Control, Mouse

MAD FL Positive Control, Mouse

SV5 Positive Control, Mouse

Rotavirus Positive Control, Mouse

Hanta Virus Positive Control, Mouse

Mycoplasma pulmonis Positive Control, Mouse

MNV Positive Control, Mouse

Rat Coronavirus Positive Control

Reo-3 Positive Control, Rat

Rat Thielovirus Positive Control

PVM Positive Control, Rat

Sendai Virus Positive Control, Rat

LCMV Positive Control, Rat

MAD K87 Positive Control, Rat

MAD FL Positive Control, Rat

Hanta Virus Positive Control, Rat

Rat Parvovirus Positive Control

Mycoplasma pulmonis Positive control, Rat

Reo-3 Positive Control, Hamster

PVM Positive Control, Hamster

Sendai Virus Positive Control, Hamster

Reo-3 Positive Control, Guinea Pig

TMEV Positive Control, Guinea Pig

PVM Positive Control, Guinea Pig

Sendai Virus Positive Control, Guinea Pig

Guine Pig Adenovirus Positive Control

Guine Pig CMV Positive Control

PIV3 Positive Control, Guinea Pig

Reo-3 Positive Control, Rabbit

PVM Positive Control, Rabbit

Sendai Virus Positive Control, Rabbit

SV5 Positive Control, Rabbit

Rotavirus Positive Control, Rabbit

Myxomatosis Positive Control, Rabbit

Pack size

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

1ml

BioDoc IFA Negative controls

Reference #

IFA-NC-M

IFA-NC-R

IFA-NC-H

IFA-NC-GP

IFA-NC-RB

Species

Mouse IFA Negative Control

Rat IFA Negative Control

Hamster IFA Negative Control

Guinea Pig IFA Negative Control

Rabbit IFA Negative Control

Pack size

1ml

1ml

1ml

1ml

1ml

BioDoc IFA Reagents

Reference #

IFA-FC-M

IFA-FC-R

IFA-FC-H

IFA-FC-GP

IFA-FC-RB

IFA-Block

IFA-PBS

IFA-SCD

IFA-Mount

IFA-CS

IFA-Blot

Species

Fluorescein Conjugate (1000X), Mouse

Fluorescein Conjugate (1000X), Rat

Fluorescein Conjugate (1000X), Hamster

Fluorescein Conjugate (1000X), Guinea Pig

Fluorescein Conjugate (1000X), Rabbit

Blocking Buffer

Phosphate Buffer Saline (PBS)

Sample/Conjugate Diluent Buffer

Mounting Solution

Slide Cover Slips 

10-Well Blotters

Pack size

10 µl

10 µl

10 µl

10 µl

10 µl

1ml

1L

10ml

1ml

12

15

Prof. Mälher selected bibliography
  • Mähler M, Köhl W. 2009. A serological survey to evaluate contemporary prevalence of viral agents and Mycoplasma pulmonis in laboratory mice and rats in western Europe. Lab Anim. (NY) 38(5), 161-165. 

https://www.nature.com/articles/laban0509-161

  • Dammann P, Hilken G, Hueber B, Köhl W, Bappert MT, Mähler M. 2011. Infectious microorganisms in mice (Mus musculus) purchased from commercial pet shops in Germany. Lab. Anim. 45, 271-275. 

http://journals.sagepub.com/doi/pdf/10.1258/la.2011.010183

  • Mähler M, Nicklas W. 2012. Health management and monitoring. In: Hedrich HJ (ed), Handbook of Experimental Animals: The Laboratory Mouse (2nd edn). Elsevier Academic Press, London, pp. 601-620.

  • Nicklas W, Bleich A, Mähler M. 2012. Viral Infections. In: Hedrich HJ (ed), Handbook of Experimental Animals: The Laboratory Mouse (2nd edn). Elsevier Academic Press, London, pp. 427-480. 

  • Mähler M, Berard M, Feinstein R, Gallagher A, Illgen-Wilcke B, Pritchett-Corning K, Raspa M. 2014. FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units. Lab. Anim. 48, 178-192. 

http://journals.sagepub.com/doi/pdf/10.1177/0023677213516312