ImmunoFluorescence (BioDoc production)
Prof. Dr. Michael Mähler is owner and managing director of BioDoc in the Medical Park Hannover.
His professional experience is further reflected by teaching assignments at Medical School Hannover and RWTH Aachen University, preparation of peer reviews for scientific journals and active participation in several scientific organisations.
Pathogens
Species
Catalog Ref.
BioDoc IFA slides
MNV
Mouse hepatitis virus (MHV) / Rat coronaviruses (using MHV as antigen)
Reovirus type 3 (Reo3)
Theiler’s murine encephalomyeltitis virus (TMEV) / Rat theilovirus (using TMEV as antigen)
Pneumonia virus of mice (PVM)
Sendai virus (Sendai)
Minute virus of mice (MVM)
Ectromelia virus (Ectro)
Lymphocytic chroriomeningitis virus (LCMV)
Mouse adenovirus type 1 (K87)
Mouse adenovirus type 2 (FL)
Guinea pig adenovirus (detected by using K87)
Simian Virus 5 (SV5)
Rotavirus (EDIM)
Rat parvoviruses (RPV) (detected by using Kilham rat virus as antigen)
Myxomatosis virus
Guinea pig cytomegalovirus (CMV)
Parainfluenza virus type 3 (PI3)
Murine norovirus (MNV)
Hantavirus New
Mycoplasma pulmonis New
M / R
M, R, H, Gp, Rb
M, Gp / R
M, R, H, Gp, Rb
M, R, H, Gp, Rb
M
M
M, H, Gp
M, R
M, R
Gp
H, Gp, Rb
M, Rb (not rats!)
R
Rb
Gp
Gp
M
M, R
M, R, H, GP
M = mouse
R = rat
H = hamster
Gp = guinea pig
Rb = rabbit
IFA-MHV-S202 / IFA-MHV-S202R
IFA-Reo3-S203
IFA-TMEV-S204 / IFA-TMEV-S204R
IFA-PVM-S205
IFA-Sendai-S206
IFA-MVM-S207
IFA-Ectro-S208
IFA-LCMV-S209
IFA-K87-S210
IFA-FL-S211
IFA-K87-S212
IFA-SV5-S213
IFA-Rota-S214
IFA-KRV-S215
IFA-Myxo-S216
IFA-GPCMV-S217
IFA-PIV3-S218
IFA-MNV-S219
IFA-HANT-S220
IFA-PULM-S221
Reo3
K87
Ectro
MHV
TMEV
SV5
SV5
MVM
Sendai
BioDoc IFA Positive controls
Reference #
IFA-PC202M
IFA-PC203M
IFA-PC204M
IFA-PC205M
IFA-PC206M
IFA-PC207M
IFA-PC208M
IFA-PC209M
IFA-PC210M
IFA-PC211M
IFA-PC213M
IFA-PC214M
IFA-PC220M
IFA-PC221M
IFA-PC219M
IFA-PC202R
IFA-PC203R
IFA-PC204R
IFA-PC205R
IFA-PC206R
IFA-PC209R
IFA-PC210R
IFA-PC211R
IFA-PC220R
IFA-PC215R
IFA-PC221R
IFA-PC203H
IFA-PC205H
IFA-PC206H
IFA-PC203GP
IFA-PC204GP
IFA-PC205GP
IFA-PC206GP
IFA-PC210GP
IFA-PC217GP
IFA-PC218GP
IFA-PC203RB
IFA-PC205RB
IFA-PC206RB
IFA-PC213RB
IFA-PC214RB
IFA-PC216RB
Infectious Agent / Disease
MHV Positive Control, Mouse
Reo-3 Positive Control, Mouse
TMEV Positive Control, Mouse
PVM Positive Control, Mouse
Sendai Virus Positive Control, Mouse
MVM Positive Control, Mouse
Ectromelia Virus Positive Control, Mouse
LCMV Positive Control, Mouse
MAD K87 Positive Control, Mouse
MAD FL Positive Control, Mouse
SV5 Positive Control, Mouse
Rotavirus Positive Control, Mouse
Hanta Virus Positive Control, Mouse
Mycoplasma pulmonis Positive Control, Mouse
MNV Positive Control, Mouse
Rat Coronavirus Positive Control
Reo-3 Positive Control, Rat
Rat Thielovirus Positive Control
PVM Positive Control, Rat
Sendai Virus Positive Control, Rat
LCMV Positive Control, Rat
MAD K87 Positive Control, Rat
MAD FL Positive Control, Rat
Hanta Virus Positive Control, Rat
Rat Parvovirus Positive Control
Mycoplasma pulmonis Positive control, Rat
Reo-3 Positive Control, Hamster
PVM Positive Control, Hamster
Sendai Virus Positive Control, Hamster
Reo-3 Positive Control, Guinea Pig
TMEV Positive Control, Guinea Pig
PVM Positive Control, Guinea Pig
Sendai Virus Positive Control, Guinea Pig
Guine Pig Adenovirus Positive Control
Guine Pig CMV Positive Control
PIV3 Positive Control, Guinea Pig
Reo-3 Positive Control, Rabbit
PVM Positive Control, Rabbit
Sendai Virus Positive Control, Rabbit
SV5 Positive Control, Rabbit
Rotavirus Positive Control, Rabbit
Myxomatosis Positive Control, Rabbit
Pack size
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
1ml
BioDoc IFA Negative controls
Reference #
IFA-NC-M
IFA-NC-R
IFA-NC-H
IFA-NC-GP
IFA-NC-RB
Species
Mouse IFA Negative Control
Rat IFA Negative Control
Hamster IFA Negative Control
Guinea Pig IFA Negative Control
Rabbit IFA Negative Control
Pack size
1ml
1ml
1ml
1ml
1ml
BioDoc IFA Reagents
Reference #
IFA-FC-M
IFA-FC-R
IFA-FC-H
IFA-FC-GP
IFA-FC-RB
IFA-Block
IFA-PBS
IFA-SCD
IFA-Mount
IFA-CS
IFA-Blot
Species
Fluorescein Conjugate (1000X), Mouse
Fluorescein Conjugate (1000X), Rat
Fluorescein Conjugate (1000X), Hamster
Fluorescein Conjugate (1000X), Guinea Pig
Fluorescein Conjugate (1000X), Rabbit
Blocking Buffer
Phosphate Buffer Saline (PBS)
Sample/Conjugate Diluent Buffer
Mounting Solution
Slide Cover Slips
10-Well Blotters
Pack size
10 µl
10 µl
10 µl
10 µl
10 µl
1ml
1L
10ml
1ml
12
15
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Mähler M, Köhl W. 2009. A serological survey to evaluate contemporary prevalence of viral agents and Mycoplasma pulmonis in laboratory mice and rats in western Europe. Lab Anim. (NY) 38(5), 161-165.
https://www.nature.com/articles/laban0509-161
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Dammann P, Hilken G, Hueber B, Köhl W, Bappert MT, Mähler M. 2011. Infectious microorganisms in mice (Mus musculus) purchased from commercial pet shops in Germany. Lab. Anim. 45, 271-275.
http://journals.sagepub.com/doi/pdf/10.1258/la.2011.010183
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Mähler M, Nicklas W. 2012. Health management and monitoring. In: Hedrich HJ (ed), Handbook of Experimental Animals: The Laboratory Mouse (2nd edn). Elsevier Academic Press, London, pp. 601-620.
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Nicklas W, Bleich A, Mähler M. 2012. Viral Infections. In: Hedrich HJ (ed), Handbook of Experimental Animals: The Laboratory Mouse (2nd edn). Elsevier Academic Press, London, pp. 427-480.
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Mähler M, Berard M, Feinstein R, Gallagher A, Illgen-Wilcke B, Pritchett-Corning K, Raspa M. 2014. FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units. Lab. Anim. 48, 178-192.
http://journals.sagepub.com/doi/pdf/10.1177/0023677213516312